iSIM Super Resolution

Resolution enhancement without the need for special fluorophores. Nikon has joined forces with Visitech to provide an imaging platform that can image deep in to tissue (up to 120µm) and acheive some remarkably detailed images

Imaging System

Vt-iSIM scan head with Hamamatsu Flash4.0 sCMOS camera

Objectives

       [Oil - 60X/1.4NA, 100X/1.49NA - TIRF]    [Silicon - 100X/1.4NA]

Laser Wavelength

405nm, 488nm, 561nm, 640nm

Incubation

Okolab incubator with CO2 control

Temp: 3°C above ambient to 45°C

Instructions

Advanced light microscopy Neuroscience, Fluorescence Microscopy, Light Microscopy Facility, Science

High Content Imaging, Opera Phenix, Advanced light microscopy Neuroscience, Fluorescence Microscopy, Light Microscopy Facility, Science

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Nick Gatford

Live-cell super-resolution montage of actin dynamics in a human neuronal growth cone