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iSIM Super Resolution
Resolution enhancement without the need for special fluorophores. Nikon and Visitech provide an imaging platform that can image deep in to tissue (as deep as 120µm) and achieve some remarkably detailed images (resolution ~120nm lateral & ~350nm axial)
Typical Usage
High Resolution Imaging of fixed or live cells, 3D fixed tissue imaging, rapid high detail tissue scanning
Imaging System
Vt-iSIM with Hamamatsu Flash4.0 sCMOS camera
Objectives
10x air, 20x air, 60x oil, 100x oil & 100x Silicon
Laser Wavelength
405nm, 445nm, 488nm, 514nm, 561nm, 640nm
Incubation
Okolab incubator with CO2 control
Temp: 3°C above ambient to 45°C
iSIM_tiled_Tissue
Tissue scanning (Sara Tacconelli)
Autism iPSC cell
Nick Gatford's winning image of the growing neuronal stem cell
Nick Gatford
Live-cell super-resolution montage of actin dynamics in a human neuronal growth cone
iSIM_tiled_Tissue
Tissue scanning (Sara Tacconelli)
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Instructions:
User Guide for the iSIM
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