iSIM Super Resolution

Resolution enhancement without the need for special fluorophores. Nikon and Visitech to provide an imaging platform that can image deep in to tissue (as deep as 120µm) and achieve some remarkably detailed images (resolution ~120nm lateral & ~350nm axial)

Imaging System

Vt-iSIM with Hamamatsu Flash4.0 sCMOS camera

Objectives

       60x 100x oil & 100x Silicon

Laser Wavelength

405nm, 445nm, 488nm, 514nm, 561nm, 640nm

Incubation

Okolab incubator with CO2 control

Temp: 3°C above ambient to 45°C

Autism iPSC cell
Autism iPSC cell

Nick Gatford's winning image of the growing neuronal stem cell

Sparkie
Sparkie

Nice looking Neuron imaged by Dr Elizabeth Glennon

Nick Gatford
Nick Gatford

Live-cell super-resolution montage of actin dynamics in a human neuronal growth cone

Autism iPSC cell
Autism iPSC cell

Nick Gatford's winning image of the growing neuronal stem cell

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Instructions:

User Guide to using the iSIM

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