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iSIM Super Resolution

Resolution enhancement without the need for special fluorophores. Nikon and Visitech provide an imaging platform that can image deep in to tissue (as deep as 120µm) and achieve some remarkably detailed images (resolution ~120nm lateral & ~350nm axial)

Typical Usage

High Resolution Imaging of fixed or live cells, 3D fixed tissue imaging, rapid high detail tissue scanning

Imaging System

Vt-iSIM with Hamamatsu Flash4.0 sCMOS camera


10x air, 20x air, 60x oil, 100x oil & 100x Silicon

Laser Wavelength

405nm, 445nm, 488nm, 514nm, 561nm, 640nm


Okolab incubator with CO2 control

Temp: 3°C above ambient to 45°C

User Guide for the iSIM

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Wohl Cellular Imaging Centre
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